Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody catalog # P00135. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Phospho Site

S209

Synonyms

Eukaryotic translation initiation factor 4E;eIF-4E;eIF4E;eIF-4F 25 kDa subunit;mRNA cap-binding protein;EIF4E;EIF4EL1, EIF4F;

Gene Name

Eukaryotic translation initiation factor 4E

UniProt

P06730

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human Phospho-eIF4E (S209)

Clonality

Monoclonal

Clone

Clone: DBD-5

Tissue Specificity

Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). .

Applications

WB,IHC,ICC,IF,IP

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

25097 MW

Observed Molecular Weight

70, 75 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:1000-1:10000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50

Subcellular Location

Cytoplasm, P-body . Cytoplasm .

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