Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Phospho-LRRK2 (S935) Rabbit Monoclonal Antibody catalog # P00221. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.

Product Specifications

Phospho Site

S935

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2;2.7.11.1;Dardarin;LRRK2;PARK8;

Gene Name

Leucine-rich repeat serine/threonine-protein kinase 2

UniProt

Q5S007

Host

Rabbit

Reactivity

Human,Mouse

Immunogen

A synthesized peptide derived from human Phospho-LRRK2 (S935)

Clonality

Monoclonal

Clone

Clone: BFH-12

Tissue Specificity

Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. .

Applications

WB,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Positively regulates autophagy through a calcium- dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

286103 MW

Observed Molecular Weight

100 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:1000<br>ICC/IF 1:50-1:200

Subcellular Location

Membrane; Peripheral membrane protein. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome . Lysosome . Mitochondrion outer membrane . Mitochondrion inner membrane . Mitochondrion matrix . Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes. .