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Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody, Carrier-free

Selected Variant

P00026-carrier-free

Size: 100 µL

Boster Bio Anti-Phospho-c-Myc (T58) Rabbit Monoclonal Antibody catalog # P00026. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Phospho Site

T58

Synonyms

Myc proto-oncogene protein;Class E basic helix-loop-helix protein 39;bHLHe39;Proto-oncogene c-Myc;Transcription factor p64;MYC;BHLHE39;

Gene Name

Myc proto-oncogene protein

UniProt

P01106

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-c-Myc (T58)

Clonality

Monoclonal

Clone

Clone: FOC-13

Tissue Specificity

Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. .

Applications

WB,ICC,IF,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'- CAC[GA]TG-3'. Activates the transcription of growth-related genes.

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48804 MW

Observed Molecular Weight

65 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>ICC/IF 1:50-1:200<br>FC 1:50

Subcellular Location

Nucleus, nucleoplasm . Nucleus, nucleolus .

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