Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody catalog # P00250. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Phospho Site

S326

Synonyms

Heat shock factor protein 1;HSF 1;Heat shock transcription factor 1;HSTF 1;HSF1;HSTF1;

Gene Name

Heat shock factor protein 1

UniProt

Q00613

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-HSF1 (S326)

Clonality

Monoclonal

Clone

Clone: BHI-8

Tissue Specificity

Predominantly expressed in B-lymphocytes.

Applications

WB,IHC,ICC,IF,IP,Flow Cytometry

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57260 MW

Observed Molecular Weight

80 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200<br>IP 1:50<br>FC 1:50

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.