Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15), Carrier-free

Boster Bio Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) catalog # MA1115. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat.

Product Specifications

Background

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Synonyms

Actin, cytoplasmic 1;Beta-actin;Actin, cytoplasmic 1, N-terminally processed;Actb;

Gene Name

Actin, cytoplasmic 1

Gene ID

Human(60), Mouse(11461), Rat(81822)

UniProt

P60711

Host

Mouse

Reactivity

Chicken,Human,Monkey,Mouse,Pig,Rabbit,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Clonality

Monoclonal

Clone

Clone: AC-15

Tissue Specificity

GGA1: Ubiquitously expressed.

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985. 2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006. 3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

41737 MW

Observed Molecular Weight

42 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.05-0.1μg/ml, Human, mouse, rat, pig, monkey, rabbit, chicken<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat, By Heat <br>