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Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1), Carrier-free

Boster Bio Anti-Vinculin VCL Antibody (Monoclonal, hVIN-1) catalog # MA1103. Tested in IF, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Rat.

Product Specifications

Background

Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23.

Synonyms

Vinculin ;Metavinculin ;Vcl ;

Gene Name

Vinculin

Gene ID

Human(7414), Mouse(22330), Rat(305679)

UniProt

P85972

Host

Mouse

Reactivity

Chicken,Human,Monkey,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human vinculin, purified from uterus.

Clonality

Monoclonal

Clone

Clone: hVIN-1

Tissue Specificity

VCL: Metavinculin is muscle-specific.

Applications

IF,IHC-F,ICC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity). .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Weller, P. A.; Ogryzko, E. P.; Corben, E. B.; Zhidkova, N. I.; Patel, B.; Price, G. J.; Spurr, N. K.; Koteliansky, V. E.; Critchley, D. R. : Complete sequence of human vinculin and assignment of the gene to chromosome 10. Proc. Nat. Acad. Sci. 87: 5667-5671, 1990. 2. Mulligan, L. M.; Gardner, E.; Telenius, H.; Ponder, B. A. J. : Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q. Genomics 13: 1347-1349, 1992.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

116615 MW

Observed Molecular Weight

124 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 1-2μg/ml, Human, chicken, monkey, mouse, rat<br> Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, chicken, mouse, rat, -<br> Immunocytochemistry/Immunofluorescence, 2μg/ml, Human<br>

Subcellular Location

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .

Sequence Similarities

Belongs to the vinculin/alpha-catenin family.

Protein Name

Vinculin

Frequently Asked Questions

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