Anti-Vimentin Antibody (Monoclonal, V9), Carrier-free

Boster Bio Anti-Vimentin Antibody (Monoclonal, V9) catalog # MA1102. Tested in IF, IHC, WB applications. This antibody reacts with Human, Pig, Rabbit, Rat.

Product Specifications

Background

The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.

Synonyms

Vimentin;Vim;

Gene Name

Vimentin

Gene ID

Human(7431), Mouse(22352), Rat(81818)

UniProt

P31000

Host

Mouse

Reactivity

Human,Pig,Rabbit,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Pig eye lens vimentin.

Clonality

Monoclonal

Clone

Clone: V9

Tissue Specificity

VIM: Highly expressed in fibroblasts, someexpression in T- and B-lymphocytes, and little or no expression inBurkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Applications

IF,IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Lilienbaum, A.; Legagneux, V.; Portier, M.-M.; Dellagi, K.; Paulin, D. : Vimentin gene: expression in human lymphocytes and in Burkitt's lymphoma cells. EMBO J. 5: 2809-2814, 1986. 2. Mor-Vaknin, N.; Punturieri, A.; Sitwala, K.; Markovitz, D. M. : Vimentin is secreted by activated macrophages. Nature Cell Biol. 5: 59-63, 2003 3. Ferrari, S.; Cannizzaro, L. A.; Battini, R.; Huebner, K.; Baserga, R. : The gene encoding human vimentin is located on the short arm of chromosome 10. Am. J. Hum. Genet. 41: 616-626, 1987.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53733 MW

Observed Molecular Weight

54 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.5-1μg/ml, Human, rabbit, pig, rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, rabbit, pig, rat, By Heat<br> Immunofluorescence, 5μg/ml, Human