Anti-Talin TLN2 Antibody (Monoclonal, 8d4), Carrier-free

Boster Bio Anti-Talin TLN2 Antibody (Monoclonal, 8d4) catalog # MA1092. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Product Specifications

Background

Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Talin serves a function of linking vinculin to the integrins, and, thus, the cytoskeleton to extracellular matrix (ECM) receptors. It has a mass of 270 kD and shares 23% N-terminal identity with ezrin, which has similar functions. TLN gene is mapped to 9p.

Synonyms

Protein Tln2 ;Tln2 ;

Gene Name

talin 1; talin 2

Gene ID

Human(7094), Mouse(21894), Rat(313494), Human(83660), Mouse(70549), Rat(315776)

UniProt

D3ZA84

Host

Mouse

Reactivity

Chicken,Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Purified chicken gizzard talin.

Clonality

Monoclonal

Clone

Clone: 8d4

Tissue Specificity

Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Applications

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

TLN1: Probably involved in connections of major cytoskeletalstructures to the plasma membrane. High molecular weightcytoskeletal protein concentrated at regions of cell-substratumcontact and, in lymphocytes, at cell-cell contacts (Bysimilarity). |TLN2: As a major component of focal adhesion plaques thatlinks integrin to the actin cytoskeleton, may play an importantrole in cell adhesion. Recruits PIP5K1C to focal adhesion plaquesand strongly activates its kinase activity (By similarity).

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Rees, D. J. G.; Ades, S. E.; Singer, S. J.; Hynes, R. O. : Sequence and domain structure of talin. Nature 347: 685-689, 1990. 2. Gilmore, A. P.; Ohanian, V.; Spurr, N. K.; Critchley, D. R. : Localisation of the human gene encoding the cytoskeletal protein talin to chromosome 9p. Hum. Genet. 96: 221-224, 1995.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

271684 MW

Observed Molecular Weight

272 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.5-1μg/ml, Human, mouse, rat, chicken<br>

Subcellular Location

TLN1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface Cell junction, focaladhesion Note: Colocalizes with LAYN at the membraneruffles. Localized preferentially in focal adhesions thanfibrillar adhesions (By similarity). |Tln1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface |TLN2: Cell junction, focal adhesion. Celljunction, synapse. Cell membrane; Peripheral membrane protein;Cytoplasmic side. Cytoplasm, cytoskeleton. Note: Focal adhesionplaques and synapses.|Tln2: Cell junction, focal adhesion Cell junction, synapse Cell membrane Cytoplasm, cytoskeleton Note: Focaladhesion plaques and synapses.