Anti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1), Carrier-free

Boster Bio Anti-S-100 (Beta-subunit) S100b Antibody (Monoclonal, SH-B1) catalog # MA1088. Tested in IHC applications. This antibody reacts with Human, Rabbit, Rat.

Product Specifications

Background

S100 protein is a 21,000-Da component first isolated from brain by Moore (1965). Immunocytochemical studies demonstrated that S100 protein is produced by a wide variety of normal and neoplastic cells of mesodermal, neuroectodermal, and epithelial origin (Herrera et al., 1988). The S100 molecule is a dimer; thus there are 3 forms of S100: alpha-alpha, known as S-100a (0); alpha-beta, known as S-100a; and beta-beta, known as S-100b. S100 genes are clustered on human chromosome 1q21. S-100 protein expression by primary and metastatic adenocarcinomas.

Synonyms

Protein S100-B;S-100 protein beta chain;S-100 protein subunit beta;S100 calcium-binding protein B;S100b;

Gene Name

Protein S100-B

Gene ID

Human(6285), Mouse(20203), Rat(25742)

UniProt

P04631

Host

Mouse

Reactivity

Human,Rabbit,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Bovine brain S-100b.

Clonality

Monoclonal

Clone

Clone: SH-B1

Tissue Specificity

Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. .

Applications

IHC

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity. .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Engelkamp, D.; Schafer, B. W.; Mattei, M. G.; Erne, P.; Heizmann, C. W. : Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E. Proc. Nat. Acad. Sci. 90: 6547-6551, 1993. 2. Herrera, G. A.; Turbat-Herrera, E. A.; Lott, R. L. : S-100 protein expression by primary and metastatic adenocarcinomas. Am. J. Clin. Path. 89: 168-176, 1988.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.