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Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10), Carrier-free

Selected Variant

MA1080-carrier-free

Size: 100 µg

Boster Bio Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) catalog # MA1080. Tested in ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Product Specifications

Background

The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.

Synonyms

Paxillin;Pxn;

Gene Name

Paxillin

Gene ID

Human(5829), Mouse(19303), Rat(360820)

UniProt

Q66H76

Host

Mouse

Reactivity

Bovine,Chicken,Hamster,Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

C-terminal part of recombinant chicken paxillin (amino acids 305-559).

Clonality

Monoclonal

Clone

Clone: PXC-10

Tissue Specificity

CDH1: Non-neural epithelial tissues.

Applications

ICC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Salgia, R.; Li, J.-L.; Lo, S. H.; Brunkhorst, B.; Kansas, G. S.; Sobhany, E. S.; Sun, Y.; Pisick, E.; Hallek, M.; Ernst, T.; Tantravahi, R.; Chen, L. B.; Griffin, J. D. : Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210(BCR/ABL). J. Biol. Chem. 270: 5039-5047, 1995. 2. Turner, C. E.; Glenney, J. R., Jr.; Burridge, K. : Paxillin: a new vinculin-binding protein present in focal adhesions. J. Cell Biol. 111: 1059-1068, 1990.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

64019 MW

Observed Molecular Weight

65 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunocytochemistry , 1μg/ml, Human, mouse, rat, chicken, -<br>Western blot, 1-2μg/ml, Human, mouse, rat, chicken<br>

Subcellular Location

Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity). .

Sequence Similarities

Belongs to the paxillin family.

Protein Name

Paxillin

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