Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100), Carrier-free

Boster Bio Anti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) catalog # MA1075. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Product Specifications

Background

Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin-dependent kinases. P19 (INK4d) also kowns as cyclin-dependent kinase inhibitor 2D, is one of the novel members of the mouse INK4 gene family. Okuda et al. (1995) described the cloning of the human INK4d gene (CDKN2D). The predicted 166-amino acid protein is 86% identical to the mouse protein and about 45% identical to other human INK4 family members.

Synonyms

Cyclin-dependent kinase 4 inhibitor D;p19-INK4d;Cdkn2d;

Gene Name

Cyclin-dependent kinase 4 inhibitor D

Gene ID

Human(1032), Mouse(12581), Rat(494444)

UniProt

Q60773

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Recombinant human p19INK4d.

Clonality

Monoclonal

Clone

Clone: DCS-100

Tissue Specificity

Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. .

Applications

IHC,ICC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Interacts strongly with CDK4 and CDK6 and inhibits them. .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Hirai, H.; Roussel, M. F.; Kato, J.-Y.; Ashmun, R. A.; Sherr, C. J. : Novel INK4 proteins, p19 and p18, are specific inhibitors of cyclin D-dependent kinases CDK4 and CDK6. Molec. Cell. Biol. 15: 2672-2681, 1995. 2. tsuzaki, Y.; Miyazawa, K.; Yokota, T.; Hitomi, T.; Yamagishi, H.; Sakai, T. : Molecular cloning and characterization of the human p19(INK4d) gene promoter. FEBS Lett. 517: 272-276, 2002.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

17809 MW

Observed Molecular Weight

18 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat <br>Immunocytochemistry , 1μg/ml, Human, -<br>Western blot, 0.5-1μg/ml, Human<br>