Anti-NF68 Nefl Antibody (Monoclonal, NR4), Carrier-free

Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.

Product Specifications

Background

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Synonyms

Neurofilament light polypeptide;NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein;Nefl;Nf68, Nfl;

Gene Name

Neurofilament light polypeptide

Gene ID

Human(4747), Mouse(18039), Rat(83613)

UniProt

P19527

Host

Mouse

Reactivity

Human,Mouse,Pig,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Pig spinal cord.

Clonality

Monoclonal

Clone

Clone: NR4

Tissue Specificity

Muscle specific. Located in the thin filament of striated muscle.

Applications

IHC,IHC-F,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Zhu, Q.; Couillard-Despres, S.; Julien, J.-P. : Delayed maturation of regenerating myelinated axons in mice lacking neurofilaments. Exp. Neurol. 148: 299-316, 1997. 2. Jordanova, A.; De Jonghe, P.; Boerkoel, C. F.; Takashima, H.; De Vriendt, E.; Ceuterick, C.; Martin, J.-J.; Butler, I. J.; Mancias, P.; Papasozomenos, S. C.; Terespolsky, D.; Potocki, L.; Brown, C. W.; Shy, M.; Rita, D. A.; Tournev, I.; Kremensky, I.; Lupski, J. R.; Timmerman, V. : Mutations in the neurofilament light chain gene (NEFL) cause early onset severe Charcot-Marie-Tooth disease. Brain 126: 590-597, 2003. 3. Previtali, S. C.; Zerega, B.; Sherman, D. L.; Brophy, P. J.; Dina, G.; King, R. H. M.; Salih, M. M.; Feltri, L.; Quattrini, A.; Ravazzolo, R.; Wrabetz, L.; Monaco, A. P.; Bolino, A. : Myotubularin-related 2 protein phosphatase and neurofilament light chain protein, both mutated in CMT neuropathies, interact in peripheral nerve. Hum. Molec. Genet. 12: 1713-1723, 2003.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

61335 MW

Observed Molecular Weight

72 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, pig, rat, By Heat <br>Immunohistochemistry (Frozen Section), 2-4μg/ml, Human, mouse, pig, rat, -<br>Western blot, 1-2μg/ml, Human, mouse, pig, rat<br>