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Anti-Myosin(Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D), Carrier-free

Selected Variant

MA1064-carrier-free

Size: 100 µg

Boster Bio Anti-Myosin (Skeletal, Slow) Myh7 Antibody (Monoclonal, NOQ7.5.4D) catalog # MA1064. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Product Specifications

Background

Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. Skeletal Myosin (slow),, also known as light chain 3 (MYL3), mapped to 3p. Fodor et al. (1989) found that the MYL3 gene has 7 exons, the last of which is completely untranslated 3-prime sequence.

Synonyms

Myosin-7;Myosin heavy chain 7;Myosin heavy chain slow isoform;MyHC-slow;Myosin heavy chain, cardiac muscle beta isoform;MyHC-beta;Myh7;

Gene Name

Myosin-7

Gene ID

Human(4625), Mouse(140781), Rat(29557)

UniProt

P12883

Host

Mouse

Reactivity

Human,Monkey,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human skeletal muscle myosin purified from myofibrils.

Clonality

Monoclonal

Clone

Clone: NOQ7.5.4D

Tissue Specificity

MYH7: Both wild type and variant Gln-403 aredetected in skeletal muscle (at protein level).

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Darras, B. T.; Fodor, B.; Vanin, E.; Francke, U. : A human myosin alkali light chain gene mapped to chromosome 3. (Abstract) Cytogenet. Cell Genet. 46: 603, 1987 2. Fodor, W. L.; Darras, B.; Seharaseyon, J.; Falkenthal, S.; Francke, U.; Vanin, E. F. : Human ventricular/slow twitch myosin alkali light chain gene characterization, sequence, and chromosomal location. J. Biol. Chem. 264: 2143-2149, 1989.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

223097 MW

Observed Molecular Weight

230 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.5-2μg/ml, Human, monkey, mouse, rat<br> Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, monkey, mouse, rat, By Heat <br>

Subcellular Location

Cytoplasm, myofibril. Thick filaments of the myofibrils.

Sequence Similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Protein Name

Myosin-7

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