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Anti-Myosin(Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32), Carrier-free

Selected Variant

MA1063-carrier-free

Size: 100 µg

Boster Bio Anti-Myosin (Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32) catalog # MA1063. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Product Specifications

Background

Myosin is composed of 2 heavy chains of about 200,000 daltons each and 4 light chains of about 20,000 daltons each. The light chains are of 2 distinct types: the phosphorylatable, regulatory, or MLC2 type, and the nonphosphorylatable, alkali, or MLC1 and MLC3 types (MYL1 according to the HGM symbols). Skeletal Myosin (Fast),, also known as MYL1, mapped to the region 2q32.1-qter.

Synonyms

Myh2 protein ;Myh2 ;

Gene Name

myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult

Gene ID

Human(4619), Mouse(17879), Rat(287408), Human(4620), Mouse(17882), Rat(691644)

UniProt

I6L963

Host

Mouse

Reactivity

Human,Mouse,Rabbit,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Rabbit muscle myosin.

Clonality

Monoclonal

Clone

Clone: MY-32

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

MYH1: Muscle contraction.|MYH2: Muscle contraction. Required for cytoskeletonorganization (By similarity).

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Cohen-Haguenauer, O.; Barton, P. J. R.; Van Cong, N.; Serero, S.; Gross, M.-S.; Jegou-Foubert, C.; de Tand, M.-F.; Robert, B.; Buckingham, M.; Frezal, J. : Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q32.1-2qter. Hum. Genet. 78: 65-70, 1988. 2. Serero, S.; Barton, P.; Van Cong, N.; Cohen-Haguenauer, O.; Robert, B.; Buckingham, M.; Frezal, J. : Assignment of the human fast skeletal muscle myosin alkali light chains gene (MLC1F/MLC3F) to 2q32.1-2qter. (Abstract) Cytogenet. Cell Genet. 46: 690 only, 1987.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

183084 MW

Observed Molecular Weight

200 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 1.5-2μg/ml, Human, mouse, rabbit, rat, By Heat <br>Western blot, 1-2μg/ml, Human, mouse, rabbit, rat<br>

Subcellular Location

MYH1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|Myh1: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.|MYH2: Cytoplasm, myofibril. Note: Thick filamentsof the myofibrils.

Sequence Similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Protein Name

Myosin-1; Myosin-2

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