Anti-HSP27 Hspb1 Monoclonal Antibody, Carrier-free

Boster Bio Anti-HSP27 Hspb1 Monoclonal Antibody catalog # MA1048. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Product Specifications

Background

The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.

Synonyms

Heat shock protein beta-1;HspB1;Heat shock 27 kDa protein;HSP 27;Hspb1;Hsp27;

Gene Name

Heat shock protein beta-1

Gene ID

Human(3315), Mouse(15507), Rat(24471)

UniProt

P42930

Host

Mouse

Reactivity

Human,Mouse,Rabbit,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

Clonality

Monoclonal

Clone

Clone: IAP-9

Tissue Specificity

Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles. .

Applications

IHC,ICC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Involved in stress resistance and actin organization.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Evgrafov, O. V.; Mersiyanova, I.; Irobi, J.; Van Den Bosch, L.; Dierick, I.; Leung, C. L.; Schagina, O.; Verpoorten, N.; Van Impe, K.; Fedotov, V.; Dadali, E.; Auer-Grumbach, M.; and 14 others : Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Nature Genet. 36: 602-606, 2004. 2. Wyttenbach, A.; Sauvageot, O.; Carmichael, J.; Diaz-Latoud, C.; Arrigo, A.-P.; Rubinsztein,D.C. :Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum. Molec. Genet. 11: 1137-1151, 2002.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

22893 MW

Observed Molecular Weight

50 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat <br>Immunocytochemistry , 1μg/ml, Human, mouse, rabbit, rat, -<br>Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rabbit, rat, -<br>Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat<br>

Subcellular Location

Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity). .