Anti-GFAP Antibody (Monoclonal, G-A-5), Carrier-free

Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.

Product Specifications

Background

Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

Synonyms

Glial fibrillary acidic protein;GFAP;Gfap;

Gene Name

Glial fibrillary acidic protein

Gene ID

Human(2670), Mouse(14580), Rat(24387)

UniProt

P47819

Host

Mouse

Reactivity

Human,Mouse,Pig,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

GFAP from pig spinal cord.

Clonality

Monoclonal

Clone

Clone: G-A-5

Tissue Specificity

Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment. .

Applications

IF,IHC,IHC-F,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Brenner, M.; Johnson, A. B.; Boespflug-Tanguy, O.; Rodriguez, D.; Goldman, J. E.; Messing, A. : Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nature Genet. 27: 117-120, 2001. 2. Rodriguez, D.; Gauthier, F.; Bertini, E.; Bugiani, M.; Brenner, M.; N'guyen, S.; Goizet, C.; Gelot, A.; Surtees, R.; Pedespan, J.-M.; Hernandorena, X.; Troncoso, M.; Uziel, G.; Messing, A.; Ponsot, G.; Pham-Dinh, D.; Dautigny, A.; Boespflug-Tanguy, O. : Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation. Am. J. Hum. Genet. 69: 1134-1140, 2001. Note: Erratum: Am. J. Hum. Genet. 69: 1413 only, 2001.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49957 MW

Observed Molecular Weight

50 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.5-1μg/ml, Human, mouse, pig, rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, rat, By Heat <br>Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, pig, rat, -<br> Immunofluorescence, 2μg/ml, Rat<br>