Anti-GAP43 Antibody (Monoclonal, GAP-7B10), Carrier-free

Boster Bio Anti-GAP43 Antibody (Monoclonal, GAP-7B10) catalog # MA1042. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Background

GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.

Synonyms

Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Protein F1;Gap43;

Gene Name

Neuromodulin

Gene ID

Human(2596), Mouse(14432), Rat(29423)

UniProt

P07936

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

GAP-43 from neonatal rat forebrain membranes.

Clonality

Monoclonal

Clone

Clone: GAP-7B10

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity). .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Kosik, K. S.; Orecchio, L. D.; Bruns, G. A. P.; Benowitz, L. I.; MacDonald, G. P.; Cox, D. R.; Neve, R. L. : Human GAP-43: its deduced amino acid sequence and chromosomal localization in mouse and human. Neuron 1: 127-132, 1988. 2. Strittmatter, S. M.; Fankhauser, C.; Huang, P. L.; Mashimo, H.; Fishman, M. C. : Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein GAP-43. Cell 80: 445-452, 1995.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23603 MW

Observed Molecular Weight

426 kDa

Fragment

Mouse IgG2a

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat <br>Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -<br>Western blot, 0.5-1μg/ml, Human, mouse, rat<br>

Subcellular Location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes.