Anti-Desmin Antibody (Monoclonal, DE-U-10), Carrier-free

Boster Bio Anti-Desmin Antibody (Monoclonal, DE-U-10) catalog # MA1036. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Product Specifications

Background

Desmin belongs to the type III family of intermediate filaments, a class of cytoskeletal elements. DES gene encodes desmin, a muscle-specific cytoskeletal protein found in smooth, cardiac, and heart muscles. Tidball (1992) found that desmin was codistributed with actin thin filaments within the cellular processes of myotendinous junctions in frog skeletal muscle. DES gene contains 9 exons and spans about 8.4 kb. By in situ hybridization, Viegas-Pequignot et al. (1989) localized the gene to 2q35. Desmin mutation responsible for idiopathic dilated cardiomyopathy.

Synonyms

Desmin;Des;

Gene Name

Desmin

Gene ID

Human(1674), Mouse(13346), Rat(64362)

UniProt

P48675

Host

Mouse

Reactivity

Human,Mouse,Rabbit,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Desmin from pig stomach.

Clonality

Monoclonal

Clone

Clone: DE-U-10

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Tidball, J. G. : Desmin at myotendinous junctions. Exp. Cell Res. 199: 206-212, 1992. 2. Viegas-Pequignot, E.; Lin, L. Z.; Dutrillaux, B.; Apiou, F.; Paulin, D. : Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization. Hum. Genet. 83: 33-36, 1989. 3. Li, D.; Tapscoft, T.; Gonzalez, O.; Burch, P. E.; Quinones, M. A.; Zoghbi, W. A.; Hill, R.; Bachinski, L. L.; Mann, D. L.; Roberts, R. : Desmin mutation responsible for idiopathic dilated cardiomyopathy. Circulation 100: 461-464, 1999.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53457 MW

Observed Molecular Weight

53 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rat, rabbit, By Heat <br>Western blot, 2μg/ml, Human, mouse, rat, rabbit<br>

Subcellular Location

Cytoplasm, myofibril, sarcomere, Z line . Cytoplasm . Cell membrane, sarcolemma . Localizes in the intercalated disks which occur at the Z line of cardiomyocytes. .