Anti-CD4 Antibody (Monoclonal, CA-4), Carrier-free

Boster Bio Anti-CD4 Antibody (Monoclonal, CA-4) catalog # MA1016. Tested in <a href="https://www.bosterbio.com/anti-human-cd4-apc-conjugated-flow-validated-fc00344-apc-boster.html" style="color:#EA8D28">Flow Cytometry</a>, IHC, ICC applications. This antibody reacts with Human.

Product Specifications

Background

CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

Synonyms

T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;W3/25 antigen;CD4;Cd4;

Gene Name

T-cell surface glycoprotein CD4

Gene ID

Human(920), Mouse(12504), Rat(24932)

UniProt

P05540

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Clonality

Monoclonal

Clone

Clone: CA-4

Tissue Specificity

Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .

Applications

Flow Cytometry,IHC,ICC

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Committee on Human Leukocyte Differentiation Antigens, IUIS WHO Nomenclature Subcommittee : Proposed nomenclature for human leukocyte differentiation antigens. Bull. WHO 5: 809-811, 1984. Note: Alternate: Immunology Today 5: 280 only, 1984. 2. Grakoui, A.; Bromley, S. K.; Sumen, C.; Davis, M. M.; Shaw, A. S.; Allen, P. M.; Dustin, M. L. : The immunological synapse: a molecular machine controlling T cell activation. Science 285: 221-227, 1999.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

51438 MW

Observed Molecular Weight

25 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Frozen Section), 1μg/ml, Human<br>Immunocytochemistry, 0.5-1μg/ml, Human<br> Flow Cytometry, 1-3μg/1x10<sup>6</sup> cells, Human