Anti-Calponin Cnn2 Antibody (Monoclonal, CP-93), Carrier-free

Boster Bio Anti-Calponin Cnn2 Antibody (Monoclonal, CP-93) catalog # MA1011. Tested in IHC, ICC, WB applications. This antibody reacts with Chicken, Human, Turkey.

Product Specifications

Background

Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.

Synonyms

Calponin-2;Calponin H2, smooth muscle;Neutral calponin;Cnn2;

Gene Name

Calponin-2

Gene ID

Human(1265), Mouse(12798)

UniProt

Q08093

Host

Mouse

Reactivity

Chicken,Human,Turkey

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Turkey gizzard calponin

Clonality

Monoclonal

Clone

Clone: CP-93

Tissue Specificity

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Applications

IHC,ICC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Strasser, P.; Gimona, M.; Moessler, H.; Herzog, M.; Small, J. V. : Mammalian calponin: identification and expression of genetic variants. FEBS Lett. 330: 13-18, 1993.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33156 MW

Observed Molecular Weight

33 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, chicken, turkey, By Heat <br>Immunocytochemistry , 1μg/ml, Human, chicken, turkey, -<br>Western blot, 0.25-0.5μg/ml, Human, chicken, turkey<br>

Subcellular Location

Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. .