Anti-Calmodulin Calm1 Antibody (Monoclonal, 6D4), Carrier-free

Boster Bio Anti-Calmodulin Calm1 Antibody (Monoclonal, 6D4) catalog # MA1010. Tested in WB applications. This antibody reacts with Chicken, Rat.

Product Specifications

Background

Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters. Three calmodulin genes (CALM1, CALM2, and CALM3) map to chromosomes 14q24-q31, 2p21.1-p21.3, and 19q13.2-q13.3, respectively.

Synonyms

Calmodulin;CaM;Calm1;Calm, Cam, Cam1;Calm2;Cam2, Camb;Calm3;Cam3, Camc;

Gene Name

Calmodulin

Gene ID

Human(801), Mouse(12313), Rat(24242), Human(805), Mouse(12314), Rat(12315), Human(808), Mouse(12315), Rat(50663)

UniProt

P62161

Host

Mouse

Reactivity

Chicken,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

Clonality

Monoclonal

Clone

Clone: 6D4

Tissue Specificity

High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Applications

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca (2+). Among the enzymes to be stimulated by the calmodulin-Ca (2+) complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (By similarity). .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Berchtold, M. W.; Egli, R.; Rhyner, J. A.; Hameister, H.; Strehler, E. E. : Localization of the human bona fide calmodulin genes CALM1, CALM2, and CALM3 to chromosomes 14q24-q31, 2p21.1-p21.3, and 19q13.2-q13.3. Genomics 16: 461-465, 1993.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

16838 MW

Observed Molecular Weight

61 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.5μg/ml, Chicken, rat<br>

Subcellular Location

Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. .