Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955), Carrier-free

Boster Bio Anti-Calbindin-D Calb1 Antibody (Monoclonal, CB-955) catalog # MA1007. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.

Product Specifications

Background

The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al. (1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.

Synonyms

Calbindin;Calbindin D28;D-28K;Spot 35 protein;Vitamin D-dependent calcium-binding protein, avian-type;Calb1;

Gene Name

Calbindin

Gene ID

Human(793), Mouse(12307), Rat(83839)

UniProt

P07171

Host

Mouse

Reactivity

Bovine,Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Bovine kidney calbindin-D

Clonality

Monoclonal

Clone

Clone: CB-955

Applications

IF,IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Buffers cytosolic calcium. May stimulate a membrane Ca (2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Parmentier, M.; Passage, E.; Vassart, G.; Mattei, M.-G. : The human calbindin D28k (CALB1) and calretinin (CALB2) genes are located at 8q21.3-q22.1 and 16q22-q23, respectively, suggesting a common duplication with the carbonic anhydrase isozyme loci. Cytogenet. Cell Genet. 57: 41-43, 1991.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29994 MW

Observed Molecular Weight

28 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, bovine, mouse, rat, By Heat <br>Western blot, 0.5-1μg/ml, Human, bovine, mouse, rat<br>Immunofluorescence, 2μg/ml, Mouse