TPPP/p25 Antibody

Anti brain specific protein p25 alpha antibody, anti glycogen synthase kinase 3 (GSK3) inhibitor p24 antibody, anti p24 antibody, anti p25 antibody, anti p25alpha antibody, anti TPPP/p25 antibody, anti TPPP1 antibody, anti tubulin polymerization promoting protein antibody, anti tubulin polymerization-promoting protein antibody, anti TPPP antibody

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000. WB: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313 (20) :4091-106; PMID: 18028908) . Recommended concentration: 0.001-0.01μg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2μg/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75μg/ml.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.