Anti-SLC20A1 Antibody Picoband®, Carrier-free

Boster Bio Anti-SLC20A1 Antibody Picoband® catalog # A03537-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Sodium-dependent phosphate transporter 1 is a protein that in humans is encoded by the SLC20A1 gene. The protein encoded by this gene is a sodium-phosphate symporter that absorbs phosphate from interstitial fluid for use in cellular functions such as metabolism, signal transduction, and nucleic acid and lipid synthesis. The encoded protein is also a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.

Synonyms

Segment polarity protein dishevelled homolog DVL-1;Dishevelled-1;DSH homolog 1;DVL1;

Gene Name

Segment polarity protein dishevelled homolog DVL-1

Gene ID

6574

UniProt

Q8WUM9

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SLC20A1 recombinant protein (Position: A79-K494).

Clonality

Polyclonal

Tissue Specificity

Found in embryos and in adult liver and heart.

Applications

ELISA,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Beck, L., Leroy, C., Beck-Cormier, S., Forand, A., Salaun, C., Paris, N., Bernier, A., Urena-Torres, P., Prie, D., Ollero, M., Coulombel, L., Friedlander, G. The phosphate transporter PiT1 (Slc20a1) revealed as a new essential gene for mouse liver development. PLoS One 5: e9148, 2010. 2. Inden, M., Iriyama, M., Zennami, M., Sekine, S., Hara, A., Yamada, M., Hozumi, I. The type III transporters (PiT-1 and PiT-2) are the major sodium-dependent phosphate transporters in the mice and human brains. Brain Res. 1637: 128-136, 2016. 3. Kaelbling, M., Eddy, R., Shows, T. B., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Klinger, H. P., O'Hara, B. Localization of the human gene allowing infection by gibbon ape leukemia virus to human chromosome region 2q11-q14 and to the homologous region on mouse chromosome 2. J. Virol. 65: 1743-1747, 1991.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

75187 MW

Observed Molecular Weight

85 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat<br> Immunofluorescence, 5 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytosol . Cytoplasmic vesicle . Localizes at the cell membrane upon interaction with frizzled family members. .