Anti-ALYREF Antibody Picoband®, Carrier-free

Boster Bio Anti-ALYREF Antibody Picoband® catalog # A03580-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Aly/REF export factor, also known as THO complex subunit 4 is a protein that in humans is encoded by the ALYREF gene. The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

Synonyms

Segment polarity protein dishevelled homolog DVL-3;Dishevelled-3;DSH homolog 3;DVL3;KIAA0208;

Gene Name

Segment polarity protein dishevelled homolog DVL-3

Gene ID

10189

UniProt

Q86V81

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ALYREF recombinant protein (Position: M1-S257).

Clonality

Polyclonal

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May play a role in the signal transduction pathway mediated by multiple Wnt genes.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Bruhn, L., Munnerlyn, A., Grosschedl, R. ALY, a context-dependent coactivator of LEF-1 and AML-1, is required for TCR-alpha enhancer function. Genes Dev. 11: 640-653, 1997. 2. Luo, M.-J., Zhou, Z., Magni, K., Christoforides, C., Rappsilber, J., Mann, M., Reed, R. Pre-mRNA splicing and mRNA export linked by interactions between UAP56 and Aly. Nature 413: 644-647, 2001. 3. Masuda, S., Das, R., Cheng, H., Hurt, E., Dorman, N., Reed, R. Recruitment of the human TREX complex to mRNA during splicing. Genes Dev. 19: 1512-1517, 2005.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

78055 MW

Observed Molecular Weight

35 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -