Anti-NNT Antibody Picoband®, Carrier-free

Boster Bio Anti-NNT Antibody Picoband® catalog # A00887-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD (H) and NADP (+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.

Synonyms

Glucokinase; Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D; GCK

Gene Name

glucokinase

Gene ID

23530

UniProt

Q13423

Host

Rabbit

Reactivity

Human,Mouse,Monkey,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NNT recombinant protein (Position: C44-A350).

Clonality

Polyclonal

Tissue Specificity

Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Hasselmann, C., Deladoey, J., Vuissoz, J.-M., Patry, L., Alirezaie, N., Schwartzentruber, J., FORGE Canada Consortium, Deal, C. L., Van Vliet, G., Majewski, J., Samuels, M. E. Expanding the phenotypic spectrum of nicotinamide nucleotide transhydrogenase (NNT) mutations and using whole exome sequencing to discover potential disease modifiers. J. Genomes Exomes 2: 19-30, 2013. 2. Meimaridou, E., Kowalczyk, J., Guasti, L., Hughes, C. R., Wagner, F., Frommolt, P., Nurnberg, P., Mann, N. P., Banerjee, R., Saka, H. N., Chapple, J. P., King, P. J., Clark, A. J. L., Metherell, L. A. Mutations in NNT encoding nicotinamide nucleotide transhydrogenase cause familial glucocorticoid deficiency. Nature Genet. 44: 740-742, 2012. 3. Roucher-Boulez, F., Mallet-Motak, D., Samara-Boustani, D., Jilani, H., Ladjouze, A., Souchon, P.-F., Simon, D., Nivot, S., Heinrichs, C., Ronze, M., Bertagna, X., Groisne, L., Leheup, B., Naud-Saudreau, C., Blondin, G., Lefevre, C., Lemarchand, L., Morel, Y. NNT mutations: a cause of primary adrenal insufficiency, oxidative stress and extra-adrenal defects. Europ. J. Endocr. 175: 73-84, 2016.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

114 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.25μg/ml, Human, Mouse, Monkey, Rat Immunocytochemistry/Immunofluorescence, 4μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -