Anti-NFIC/CTF Antibody Picoband®, Carrier-free

Boster Bio Anti-NFIC/CTF Antibody Picoband® catalog # A04154-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Nuclear factor 1 C-type is a protein that in humans is encoded by the NFIC gene. It is mapped to 19p13.3. The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Synonyms

Nuclear factor 1 C-type; NF1-C; Nuclear factor 1/C; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/C; NF-I/C; NFI-C; TGGCA-binding protein; NFIC; NFI

Gene Name

nuclear factor I C

Gene ID

4782

UniProt

P08651

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NFIC/CTF recombinant protein (Position: D215-G508).

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Applications

ELISA,Flow Cytometry,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Grunder, A., Qian, F., Ebel, T. T., Mincheva, A., Lichter, P., Kruse, U., Sippel, A. E. Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor nuclear factor one. Gene 304: 171-181, 2003. 2. Qian, F., Kruse, U., Lichter, P., Sippel, A. E. Chromosomal localization of the four genes (NFIA, B, C, and X) for the human transcription factor nuclear factor I by FISH. Genomics 28: 66-73, 1995. 3. Steele-Perkins, G., Butz, K. G., Lyons, G. E., Zeichner-David, M., Kim, H.-J., Cho, M.-I., Gronostajski, R. M. Essential role for NFI-C/CTF transcription-replication factor in tooth root development. Molec. Cell. Biol. 23: 1075-1084, 2003.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

90314 MW

Observed Molecular Weight

55-65 kDa, 70 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunofluorescence, 2μg/ml, Human, Mouse Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -