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Anti-Human COPE DyLight® 488 conjugated Antibody(monoclonal, 9B6), Carrier-free

Boster Bio Anti-Human COPE DyLight® 488 conjugated Antibody (monoclonal, 9B6) catalog # M04544-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Background

Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Synonyms

Coatomer subunit epsilon; Epsilon-coat protein; Epsilon-COP; COPE

Gene Name

coatomer protein complex subunit epsilon

Gene ID

11316

UniProt

O14579

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.

Clonality

Monoclonal

Clone

Clone: 9B6

Label

DyLight®488

Tissue Specificity

Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm; COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side;

Applications

Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

References & Citations

1. "Entrez Gene: COPE coatomer protein complex, subunit epsilon". 2. Shima DT, Scales SJ, Kreis TE, Pepperkok R (November 1999). "Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes". Curr. Biol. 9 (15): 821–4.

Storage Conditions

At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Observed Molecular Weight

39 kDa

Fragment

Mouse IgG2a

Specificity

No cross reactivity with other proteins.

Applications Notes

Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human

Subcellular Location

Mitochondrion intermembrane space. Loosely associated with the inner membrane.

Frequently Asked Questions

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