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Anti-Human IRS1 DyLight® 488 conjugated Antibody(monoclonal, 10I3), Carrier-free

Selected Variant

M00268-Dyl488-carrier-free

Size: 100 µg

Boster Bio Anti-Human IRS1 DyLight® 488 conjugated Antibody (monoclonal, 10I3) catalog # M00268-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Background

Insulin receptor substrate 1 (IRS-1) is a signalling adapter protein that in humans is encoded by the IRS-1 gene. It is mapped to 2q36.3. This gene exhibited no intrinsic enzyme activity, and it can serve as a docking protein involved in binding and activating other signal transduction molecules after being phosphorylated on tyrosine by insulin receptor kinase. IRS1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways PI3K/Akt and Erk MAP kinase pathways. IRS1 also has important biological function for both metabolic and mitogenic (growth promoting) pathways. In addition to those, IRS1 is a key regulator of PI3K within malignant cells.

Synonyms

Insulin receptor substrate 1; IRS-1; IRS1

Gene Name

insulin receptor substrate 1

Gene ID

3667

UniProt

P35569

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequence identity with mouse and rat IRS1, respectively.

Clonality

Monoclonal

Clone

Clone: 10I3

Label

DyLight®488

Tissue Specificity

Expressed in osteoblasts, but not in osteoclasts.

Applications

Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na<sub>2</sub>HPO<sub>4</sub>, 0.05mg NaN<sub>3</sub>.

References & Citations

1. Dearth RK, Cui X, Kim HJ, Kuiatse I, Lawrence NA, Zhang X, Divisova J, Britton OL, Mohsin S, Allred DC, Hadsell DL, Lee AV (December 2006)."Mammary Tumorigenesis and Metastasis Caused by Overexpression of Insulin Receptor Substrate 1 (IRS-1) or IRS-2". Molecular and Cellular Biology 26 (24): 9302–14. 2. Houghton, A. M., Rzymkiewicz, D. M., Ji, H., Gregory, A. D., Egea, E. E., Metz, H. E., Stolz, D. B., Land, S. R., Marconcini, L. A., Kliment, C. R., Jenkins, K. M., Beaulieu, K. A., Mouded, M., Frank, S. J., Wong, K. K., Shapiro, S. D. Neutrophil elastase-mediated degradation of IRS-1 accelerates lung tumor growth. Nature Med. 16: 219-223, 2010.

Storage Conditions

At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Observed Molecular Weight

39 kDa

Fragment

Mouse IgG2a

Specificity

No cross reactivity with other proteins.

Applications Notes

Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells, Human

Subcellular Location

ciliary basal body; cytosol; nucleus; caveola; insulin receptor complex; plasma membrane; cytoplasm; intracellular membrane-bounded organelle; Complete GO annotation on QuickGO

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