NDUFS1 Antibody
Goat polyclonal antibody to NDUFS1
Product Specifications
Product Name Alternative
Anti CI-75Kd antibody, anti complex I, mitochondrial respirato antibody, anti MGC26839 antibody, anti NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa antibody, anti NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) antibody, anti NADH-coenzyme Q reductase antibody, anti OTTHUMP00000206345 antibody, anti PRO1304 antibody, anti NDUFS1 antibody
Reactivity
Human, Mouse
Target
NDUFS1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
TEKSATYVNTEGR
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 75kDa band observed in lysates of cell lines HEK293 and NIH3T3 and in Rat Heart lysates, and approx. 80kDa in Human and Mouse Heart and in Human Skeletal Muscle lysates (calculated MW of 79.5kDa according to Human NP_004997.4 and Rat NP_001005550.1, 75.4kDa according to Human NP_001186910.1, and 79.8kDa according to Mouse NP_001153510.1) . Recommended concentration: 0.1-0.5μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Heart. Recommended concentration: 5μg/ml. Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
Molecular Weight
79.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Prediction Reactivity
Bovine, Canine, Rat
Tested Applications
ELISA, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
4719
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