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Anti-Human CD18 DyLight® 550 conjugated ITGB2 Antibody(monoclonal, 1A3/2A10), Carrier-free

Selected Variant

M00458-Dyl550-carrier-free

Size: 100 µg

Boster Bio Anti-Human CD18 DyLight® 550 conjugated ITGB2 Antibody (monoclonal, 1A3/2A10) catalog # M00458-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Background

ITGB2 (INTEGRIN, BETA-2), also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. ITGB2 is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The ITGB2 gene is mapped to 21q22.3. The expression of CD18 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of ITGB2 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although ITGB2 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.

Synonyms

Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; CD18; ITGB2; CD18; MFI7

Gene Name

integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)

Gene ID

3689

UniProt

P05107

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18.

Clonality

Monoclonal

Clone

Clone: 1A3/2A10

Label

DyLight®550

Tissue Specificity

Leukocytes.

Applications

Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed:18587400). Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).

Components

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

References & Citations

1. Abramson, J. S., Mills, E. L., Sawyer, M. K., Regelmann, W. R., Nelson, J. D., Quie, P. G. Recurrent infections and delayed separation of the umbilical cord in an infant with abnormal phagocytic cell locomotion and oxidative response during particle phagocytosis. J. Pediat. 99: 887-894, 1981. 2. Akao, Y., Utsumi, K. R., Naito, K., Ueda, R., Takahashi, T., Yamada, K. Chromosomal assignments of genes coding for human leukocyte common antigen, T-200, and lymphocyte function-associated antigen 1, LFA-1 beta subunit. Somat. Cell Molec. Genet. 13: 273-278, 1987. 3. "Entrez Gene: ITGB2 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)".

Storage Conditions

At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Observed Molecular Weight

39 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Name

Membrane cofactor protein

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