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Anti-Human ACTN3 DyLight® 550 conjugated Antibody, Carrier-free

Boster Bio Anti-Human ACTN3 DyLight® 550 conjugated Antibody catalog # A02693-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Background

Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status.

Synonyms

Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; ACTN3

Gene Name

actinin alpha 3 (gene/pseudogene)

Gene ID

89

UniProt

Q08043

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3, different from the related mouse sequence by five amino acids.

Clonality

Polyclonal

Label

DyLight®550

Tissue Specificity

Expressed only in a subset of type 2 skeletal muscle fibers.

Applications

Flow Cytometry

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Components

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

References & Citations

1. Papadimitriou ID, Papadopoulos C, Kouvatsi A, Triantaphyllidis C (April 2008). "The ACTN3 gene in elite Greek track and field athletes". Int J Sports Med 29 (4): 352–5. 2. Yang N, MacArthur DG, Gulbin JP, Hahn AG, Beggs AH, Easteal S, North K (September 2003). "ACTN3 genotype is associated with human elite athletic performance". Am. J. Hum. Genet. 73 (3): 627–31.

Storage Conditions

At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Observed Molecular Weight

39 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells

Frequently Asked Questions

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