Anti-Human ITGA2B DyLight® 488 conjugated Antibody, Carrier-free

Boster Bio Anti-Human ITGA2B DyLight® 488 conjugated Antibody catalog # A01102-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Background

Integrin alpha-IIb is a protein that in humans is encoded by the ITGA2B gene. It is mapped to 17q21.32. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibrinogen receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Synonyms

Integrin alpha-IIb; GPalpha IIb; GPIIb; Platelet membrane glycoprotein IIb; CD41; Integrin alpha-IIb heavy chain; Integrin alpha-IIb light chain, form 1; Integrin alpha-IIb light chain, form 2; ITGA2B; GP2B; ITGAB

Gene Name

integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)

Gene ID

3674

UniProt

P08514

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human ITGA2B recombinant protein (Position: L32-D263).

Clonality

Polyclonal

Label

DyLight®488

Tissue Specificity

Isoform 1 and isoform 2 are expressed in platelets and megakaryocytes, but not in reticulocytes. Not detected in Jurkat, nor in U937 cell lines (PubMed:2351656). Isoform 3 is expressed in prostate adenocarcinoma, as well as in several erythroleukemia, prostate adenocarcinoma and melanoma cell lines, including PC-3, DU-145, HEL, WM983A, WM983B and WM35. Not detected in platelets, nor in normal prostate (at protein level) (PubMed:9809974).

Applications

Flow Cytometry

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.

Components

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

References & Citations

1. "Entrez Gene: ITGA2B integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)". 2. Naik UP, Parise LV (1997). "Structure and function of platelet alpha IIb beta 3.". Curr. Opin. Hematol. 4(5): 317–22. 3. Quinn MJ, Byzova TV, Qin J et al. (2004). "Integrin alphaIIbbeta3 and its antagonism.". Arterioscler. Thromb. Vasc. Biol. 23 (6): 945–52.