Anti-Human CD2AP DyLight® 488 conjugated Antibody, Carrier-free

Boster Bio Anti-Human CD2AP DyLight® 488 conjugated Antibody catalog # A01756-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Background

CD2-associated protein is a protein that in humans is encoded by the CD2AP gene. This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein ly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.

Synonyms

CD2-associated protein; Adapter protein CMS; Cas ligand with multiple SH3 domains; CD2AP

Gene Name

CD2 associated protein

Gene ID

23607

UniProt

Q9Y5K6

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human CD2AP recombinant protein (Position: K253-K337).

Clonality

Polyclonal

Label

DyLight®488

Tissue Specificity

Widely expressed in fetal and adult tissues.

Applications

Flow Cytometry

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T- cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.

Components

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

References & Citations

1. Cochran, J. N., Rush, T., Buckingham, S. C., Roberson, E. D. The Alzheimer's disease risk factor CD2AP maintains blood-brain barrier integrity. Hum. Molec. Genet. 24: 6667-6674, 2015. 2. Lowik, M. M., Groenen, P. J. T. A., Pronk, I., Lilien, M. R., Goldschmeding, R., Dijkman, H. B., Levtchenko, E. N., Monnens, L. A., van den Heuvel, L. P. Focal segmental glomerulosclerosis in a patient homozygous for a CD2AP mutation. Kidney Int. 72: 1198-1203, 2007.

Storage Conditions

At -20℃ for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Observed Molecular Weight

39 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Flow Cytometry (Fixed), 1-3μg/1x10<sup>6</sup> cells

Subcellular Location

Cytoplasm, cytoskeleton . Cell projection, ruffle . Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase.