Anti-Human ADO DyLight® 488 conjugated Antibody, Carrier-free

Boster Bio Anti-Human ADO DyLight® 488 conjugated Antibody catalog # A02700-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Background

Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Synonyms

2-aminoethanethiol dioxygenase; Cysteamine dioxygenase; ADO; C10orf22

Gene Name

2-aminoethanethiol dioxygenase

Gene ID

84890

UniProt

Q96SZ5

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Clonality

Polyclonal

Label

DyLight®488

Tissue Specificity

Expressed only in a subset of type 2 skeletal muscle fibers.

Applications

Flow Cytometry

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Components

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

References & Citations

1. Dominy, J. E., Jr., Simmons, C. R., Hirschberger, L. L., Hwang, J., Coloso, R. M., Stipanuk, M. H. Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase.J. Biol. Chem. 282: 25189-25198, 2007.