Anti-POMP Antibody Picoband®, iFluor647 Conjugate

Boster Bio Anti-POMP Antibody Picoband® catalog # A04837-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Proteasome maturation protein is a protein that in humans is encoded by the POMP gene. The protein encoded by this gene is a molecular chaperone that binds 20S preproteasome components and is essential for 20S proteasome formation. The 20S proteasome is the proteolytically active component of the 26S proteasome complex. The encoded protein is degraded before the maturation of the 20S proteasome is complete. A variant in the 5' UTR of this gene has been associated with KLICK syndrome, a rare skin disorder.

Synonyms

NACHT, LRR and PYD domains-containing protein 2; Nucleotide-binding site protein 1; PYRIN domain and NACHT domain-containing protein 1; PYRIN-containing APAF1-like protein 2; NLRP2; NALP2; NBS1; PAN1; PYPAF2

Gene Name

NLR family pyrin domain containing 2

Gene ID

51371

UniProt

Q9Y244

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human POMP recombinant protein (Position: E9-L141).

Clonality

Polyclonal

Tissue Specificity

Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Brehm, A., Liu, Y., Sheikh, A., Marrero, B., Omoyinmi, E., Zhou, Q., Montealegre, G., Biancotto, A., Reinhardt, A., Almeida de Jesus, A., Pelletier, M., Tsai, W. L., and 31 others. Additive loss-of-function proteasome subunit mutations in CANDLE/PRAAS patients promote type I IFN production. J. Clin. Invest. 125: 4196-4211, 2015. Note: Erratum: J. Clin. Invest. 126: 795 only, 2016. 2. Chondrogianni, N., Gonos, E. S. Overexpression of hUMP1/POMP proteasome accessory protein enhances proteasome-mediated antioxidant defence. Exp. Gerontol. 42: 899-903, 2007. 3. Dahlqvist, J., Klar, J., Tiwari, N., Schuster, J., Torma, H., Badhai, J., Pujol, R., van Steensel, M. A. M., Brinkhuizen, T., Gijezen, L., Chaves, A., Tadini, G., Vahlquist, A., Dahl, N. A single-nucleotide deletion in the POMP 5-prime UTR causes a transcriptional switch and altered epidermal proteasome distribution in KLICK genodermatosis. Am. J. Hum. Genet. 86: 596-603, 2010. Note: Erratum: Am. J. Hum. Genet. 86: 655 only, 2010.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.