Anti-RNGTT Antibody Picoband®, iFluor647 Conjugate

Boster Bio Anti-RNGTT Antibody Picoband® catalog # A09317-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

mRNA-capping enzyme is a protein that in humans is encoded by the RNGTT gene. Enables mRNA guanylyltransferase activity and triphosphatase activity. Involved in 7-methylguanosine mRNA capping. Predicted to be located in nucleoplasm.

Synonyms

B-cell differentiation antigen CD72; Lyb-2; CD72; CD72

Gene Name

CD72 molecule

Gene ID

8732

UniProt

O60942

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RNGTT recombinant protein (Position: L11-D438).

Clonality

Polyclonal

Tissue Specificity

Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Plays a role in B-cell proliferation and differentiation.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Pillutla, R. C., Shimamoto, A., Furuichi, Y., Shatkin, A. J. Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively. Genomics 54: 351-353, 1998. 2. Tsukamoto, T., Shibagaki, Y., Murakoshi, T., Suzuki, M., Nakamura, A., Gotoh, H., Mizumoto, K. Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme. Biochem. Biophys. Res. Commun. 243: 101-108, 1998. 3. Yamada-Okabe, T., Doi, R., Shimmi, O., Arisawa, M., Yamada-Okabe, H. Isolation and characterization of a human cDNA for mRNA 5-prime-capping enzyme. Nucleic Acids Res. 26: 1700-1706, 1998.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

69 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -