Anti-ATP4A Antibody Picoband®, iFluor647 Conjugate

Boster Bio Anti-ATP4A Antibody Picoband® catalog # A08198-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase.

Synonyms

Zinc finger protein 42 homolog; Zfp-42; Reduced expression protein 1; REX-1; hREX-1; Zinc finger protein 754; ZFP42; REX1; ZNF754

Gene Name

ZFP42 zinc finger protein

Gene ID

495

UniProt

P20648

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ATP4A, which shares 96.2% amino acid (aa) sequence identity with mouse and rat ATP4A.

Clonality

Polyclonal

Tissue Specificity

Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.

Applications

WB,IHC,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Maeda, M., Oshiman, K.-I., Tamura, S., Futai, M. Human gastric (H(+) + K(+))-ATPase gene: similarity to (Na(+) + K(+))-ATPase genes in exon/intron organization but difference in control region. J. Biol. Chem. 265: 9027-9032, 1990. 2. Song, I., Yamada, T., Trent, J. M. Mapping of the gene encoding the alpha-subunit of the human H(+),K(+)-ATPase to chromosome 19q13.1 by fluorescent in situ hybridization. Genomics 14: 547-548, 1992.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

130 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human