Anti-Calmegin/CLGN Antibody Picoband®, iFluor647 Conjugate

Boster Bio Anti-Calmegin/CLGN Antibody Picoband® catalog # A05261-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Calmegin, also known as CLGN, is a protein which in humans is encoded by the CLGN gene. Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility.

Synonyms

Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1 factor; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA

Gene Name

MAF bZIP transcription factor A

Gene ID

1047

UniProt

O14967

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Calmegin/CLGN recombinant protein (Position: E36-D610).

Clonality

Polyclonal

Tissue Specificity

Preferentially expressed in regulatory T-cells (Tregs).

Applications

WB,IHC,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Transcription factor that activates insulin gene expression. Acts synergistically with NEUROD1/BETA2 and PDX1. Binds the insulin enhancer C1/RIPE3b element. Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Ikawa, M., Nakanishi, T., Yamada, S., Wada, I., Kominami, K., Tanaka, H., Nozaki, M., Nishimune, Y., Okabe, M. Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev. Biol. 240: 254-261, 2001. 2. Ikawa, M., Wada, I., Kominami, K., Watanabe, D., Toshimori, K., Nishimune, Y., Okabe, M. The putative chaperone calmegin is required for sperm fertility. Nature 387: 607-611, 1997. 3. Tanaka, H., Ikawa, M., Tsuchida, J., Nozaki, M., Suzuki, M., Fujiwara, T., Okabe, M., Nishimune, Y. Cloning and characterization of the human calmegin gene encoding putative testis-specific chaperone. Gene 204: 159-163, 1997.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

93 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -