Anti-Cyclophilin E/PPIE Antibody Picoband® (monoclonal, 4I9)-iFluor647 Conjugate

Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

1. Kim, J.-O., Nau, M. M., Allikian, K. A., Makela, T. P., Alitalo, K., Johnson, B. E., Kelley, M. J. Co-amplification of a novel cyclophilin-like gene (PPIE) with L-myc in small cell lung cancer cell lines. Oncogene 17: 1019-1026, 1998. 2. Mi, H., Kops, O., Zimmermann, E., Jaschke, A., Tropschug, M. A nuclear RNA-binding cyclophilin in human T cells. FEBS Lett. 398: 201-205, 1996.

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br>