Anti-UBE2D1/2/3/4 Antibody Picoband®, iFluor647 Conjugate

Boster Bio Anti-UBE2D1/2/3/4 Antibody Picoband® catalog # A04728-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Homeobox protein Hox-B1;Homeobox protein Hox-2I;HOXB1;HOX2I;

Gene Name

ubiquitin conjugating enzyme E2 D1/2/3/4

Gene ID

7321/7322/7323/51619

UniProt

P51668

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human UBE2D1/2/3/4 recombinant protein (Position: D116-M147).

Clonality

Polyclonal

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.

References & Citations

1. Gehrke, S. G., Riedel, H.-D., Herrmann, T., Hadaschik, B., Bents, K., Veltkamp, C., Stremmel, W. UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood 101: 3288-3293, 2003. 2. Gutierrez, J. A., Yu, J., Rivera, S., Wessling-Resnick, M. Functional expression cloning and characterization of SFT, a stimulator of Fe transport. J. Cell Biol. 139: 895-905, 1997. Note: Erratum: J. Cell Biol. 147: 205 only, 1999. 3. Gutierrez, J. A., Yu, J., Wessling-Resnick, M. Characterization and chromosomal mapping of the human gene for SFT, a stimulator of Fe transport. Biochem. Biophys. Res. Commun. 253: 739-742, 1998.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32193 MW

Observed Molecular Weight

17 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse, Rat ELISA, 0.1-0.5μg/ml, -