Anti-WNT7B Antibody Picoband®, iFluor647 Conjugate

Boster Bio Anti-WNT7B Antibody Picoband® catalog # A05086-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Protein Wnt-7b is a protein that in humans is encoded by the WNT7B gene. This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Among members of the human WNT family, this gene product is most similar to WNT7A protein.

Synonyms

Matrix metalloproteinase-16; MMP-16; 3.4.24.-; MMP-X2; Membrane-type matrix metalloproteinase 3; MT-MMP 3; MTMMP3; Membrane-type-3 matrix metalloproteinase; MT3-MMP; MT3MMP; MMP16; MMPX2;

Gene Name

matrix metallopeptidase 16

Gene ID

7477

UniProt

P56706

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human WNT7B recombinant protein (Position: K220-D267).

Clonality

Polyclonal

Tissue Specificity

Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Applications

WB,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Gavin, B. J., McMahon, J. A., McMahon, A. P. Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development. Genes Dev. 4: 2319-2332, 1990. 2. Huguet, E. L., McMahon, J. A., McMahon, A. P., Bicknell, R., Harris, A. L. Differential expression of human Wnt genes 2,3,4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. 3. Lobov, I. B., Rao, S., Carroll, T. J., Vallance, J. E., Ito, M., Ondr, J. K., Kurup, S., Glass, D. A., Patel, M. S., Shu, W., Morrisey, E. E., McMahon, A. P., Karsenty, G., Lang, R. A. WNT7b mediates macrophage-induced programmed cell death in patterning of the vasculature. Nature 437: 417-421, 2005.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.