Anti-GDNF Antibody Picoband®, APC Conjugate

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF is also a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. In addition to its potential role in the differentiation and survival of central nervous system neurons, it has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.

E.coli-derived human GDNF recombinant protein (Position: S78-I211). Human GDNF shares 93% amino acid (aa) sequence identity with both mouse and rat GDNF.

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. .

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

1. Lin, L.-F. H.; Doherty, D. H.; Lile, J. D.; Bektesh, S.; Collins, F. GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons. Science 260: 1130-1132, 1993. 2. Bermingham, N.; Hillermann, R.; Gilmour, F.; Martin, J. E.; Fisher, E. M. Human glial cell line-derived neurotrophic factor (GDNF) maps to chromosome 5. Hum. Genet. 96: 671-673, 1995. 3. Durbec, P.; Marcos-Gutierrez, C. V.; Kilkenny, C.; Grigoriou, M.; Wartiowaara, K.; Suvanto, P.; Smith, D.; Ponder, B.; Costantini, F.; Saarma, M.; Sariola, H.; Pachnis, V. GDNF signalling through the Ret receptor tyrosine kinase. Nature 381: 789-793, 1996.

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.