Anti-SLC12A1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-SLC12A1 Antibody catalog # PA2168. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Solute carrier family 12 (sodium/potassium/chloride transporters), member 1, also called NKCC2 is specifically found in cells of the thick ascending limb of the loop of Henle in nephrons, the basic functional units of the kidney. This gene is mapped to 15q21.1. This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. This gene plays a vital role in the regulation of ionic balance and cell volume.

Synonyms

Solute carrier family 12 member 1;Bumetanide-sensitive sodium- (potassium)-chloride cotransporter 2;Kidney-specific Na-K-Cl symporter;SLC12A1;NKCC2;

Gene Name

Solute carrier family 12 member 1

UniProt

Q13621

Host

Rabbit

Reactivity

Rat,Mouse,Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A1, identical to the related mouse sequence, different from the related rat sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Kidney specific.

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Nozu, K., Iijima, K., Kawai, K., Nozu, Y., Nishida, A., Takeshima, Y., Fu, X. J., Hashimura, Y., Kaito, H., Nakanishi, K., Yoshikawa, N., Matsuo, M. In vivo and in vitro splicing assay of SLC12A1 in an antenatal salt-losing tubulopathy patient with an intronic mutation. Hum. Genet. 126: 533-538, 2009. 2. Takahashi, N., Chernavvsky, D. R., Gomez, R. A., Igarashi, P., Gitelman, H. J., Smithies, O.Uncompensated polyuria in a mouse model of Bartter's syndrome. Proc. Nat. Acad. Sci. 97: 5434-5439, 2000.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

121450 MW

Observed Molecular Weight

55 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse<br>Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat<br>Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse<br>