Anti-NRG1 Antibody Picoband®, APC Conjugate

NRG1 (Neuregulin 1), also known as ARIA, NDF or HRGA, is a protein that in humans is encoded by the NRG1 gene. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. By in situ hybridization of a tritium-labeled probe to human metaphase spreads, Orr-Urtreger et al. (1993) localized the NDF gene to 8p21-p12. In mouse embryos 14.5 days postcoitum, Orr-Urtreger et al. (1993) found that NDF expression is confined predominantly to the central and peripheral nervous systems, including the neuroepithelium that lines the lateral ventricles of the brain, the ventral horn of the spinal cord, and the intestinal as well as dorsal root ganglia.

Pro-neuregulin-1, membrane-bound isoform;Pro-NRG1;Neuregulin-1;Acetylcholine receptor-inducing activity;ARIA;Breast cancer cell differentiation factor p45;Glial growth factor;Heregulin;HRG;Neu differentiation factor;Sensory and motor neuron-derived factor;NRG1;GGF, HGL, HRGA, NDF, SMDF;

A synthetic peptide corresponding to a sequence at the C-terminus of human NRG1, different from the related rat and mouse sequences by one amino acids.

Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. .

ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. .

1. Fazzari, P., Paternain, A. V., Valiente, M., Pla, R., Lujan, R., Lloyd, K., Lerma, J., Marin, O., Rico, B. Control of cortical GABA circuitry development by Nrg1 and ErbB4 signalling. Nature 464: 1376-1380, 2010. 2. Orr-Urtreger, A., Trakhtenbrot, L., Ben-Levy, R., Wen, D., Rechavi, G., Lonai, P., Yarden, Y. Neural expression and chromosomal mapping of NEU differentiation factor to 8p12-p21. Proc. Nat. Acad. Sci. 90: 1867-1871, 1993. 3. Steinthorsdottir, V., Stefansson, H., Ghosh, S., Birgisdottir, B., Bjornsdottir, S., Fasquel, A. C., Olafsson, O., Stefansson, K., Gulcher, J. R. Multiple novel transcription initiation sites for NRG1. Gene 342: 97-105, 2004.

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat <br>Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat<br>Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse<br>Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse<br>