Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-B Raf/BRAF Antibody Picoband®, APC Conjugate

Boster Bio Anti-B Raf/BRAF Antibody catalog # PA1848. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.

Synonyms

Serine/threonine-protein kinase B-raf;2.7.11.1;Proto-oncogene B-Raf;p94;v-Raf murine sarcoma viral oncogene homolog B1;BRAF;BRAF1, RAFB1;

Gene Name

Serine/threonine-protein kinase B-raf

UniProt

P15056

Host

Rabbit

Reactivity

Rat,Mouse,Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human B Raf, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Brain and testis.

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Eychene, A., Barnier, J. V., Apiou, F., Dutrillaux, B., Calothy, G. Chromosomal assignment of two human B-raf(Rmil) proto-oncogene loci: B-raf-1 encoding the p94(Braf/Rmil) and B-raf-2, a processed pseudogene.Oncogene 7: 1657-1660, 1992. 2. Hishinuma, A., Fukata, S., Kakudo, K., Murata, Y., Ieiri, T. High incidence of thyroid cancer in long-standing goiters with thyroglobulin mutations. Thyroid 15: 1079-1084, 2005. 3. Sithanandam, G., Kolch. W., Duh, F.-M., Rapp, U. R.Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.Oncogene 5: 1775-1780, 1990.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=PA1848

Product MSDS

https://www.bosterbio.com/msds?sku=PA1848

Calculated Molecular Weight

84437 MW

Observed Molecular Weight

46 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse<br>

Subcellular Location

Nucleus . Cytoplasm. Cell membrane . Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. .

Sequence Similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Protein Name

Serine/threonine-protein kinase B-raf

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Recombinant Escherichia coli Inner membrane protein yedI (yedI), partial
MBS7060794 Inquire

Recombinant Escherichia coli Inner membrane protein yedI (yedI), partial

Ask
View Details
LCK Antibody
MBS9610984-01 0.05 mL

LCK Antibody

Ask
View Details
LCK Antibody
MBS9610984-02 0.1 mL

LCK Antibody

Ask
View Details
LCK Antibody
MBS9610984-03 0.2 mL

LCK Antibody

Ask
View Details
LCK Antibody
MBS9610984-04 5x 0.2 mL

LCK Antibody

Ask
View Details
GPX2 Antibody - middle region : FITC (ARP42018_P050-FITC)
ARP42018_P050-FITC 100 µL

GPX2 Antibody - middle region : FITC (ARP42018_P050-FITC)

Ask
View Details