Anti-NEI3 Antibody

Rabbit Polyclonal Antibody specific to NE13

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.2.2.-, EC 4.2.99.18, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3

Gene Name

NEIL3

NCBI Gene ID

UniProt

Q8TAT5

Accession Number

NP_060718

Cellular Locus

Nucleus, Chromosome

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acids 164-177 (LRAESEVKKQKGRMLG) of human NEI3 conjugated to KLH at the C- terminus.

Target Antigen

Endonuclease 8-like 3

Target

NEI3

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

DNA

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA) (PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964). Mediates interstrand cross-link repair in response to replication stress: acts by mediating DNA glycosylase activity, cleaving one of the two N-glycosyl bonds comprising the interstrand cross-link, which avoids the formation of a double-strand break but generates an abasic site that is bypassed by translesion synthesis polymerases (By similarity). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also rUniProtKB:A0A1L8HU22, PubMed:12433996, PubMed:19170771, PubMed:22569481, PubMed:23755964}.

Additionnal Information

Immunoblotting: use at 1:500 dilution. A band of approximately 68 kDa is detected. <br><br>Positive control: human testes tissue lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human NEI3 protein. Sequence homology to mouse and rat is 85%.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Prokaryotes and lower eukaryotes possess redundant activities that remove the oxidative DNA base damages produced during normal oxidative metabolism and which have been associated with cancer and aging. Thus far, only one oxidized pyrimidine- specific DNA glycosylase has been identified in humans, hNthl. NEI3 is one of three new putative human DNA glycosylases.