Anti-Fas Antibody Picoband®, APC Conjugate

Boster Bio Anti-Fas Antibody catalog # PA1119. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.

Synonyms

Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;Fas;Apt1, Tnfrsf6;

Gene Name

Tumor necrosis factor receptor superfamily member 6

UniProt

Q63199

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest levels in heart, brain, and skeletal muscle. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity). .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Desbarats, J.; Birge, R. B.; Mimouni-Rongy, M.; Weinstein, D. E.; Palerme, J.-S.; Newell, M. K. : Fas engagement induces neurite growth through ERK activation and p35 upregulation. Nature Cell Biol. 5: 118-125, 2003. 2. Arscott, P. L.; Stokes, T.; Myc, A.; Giordano, T. J.; Thompson, N. W.; Baker, J. R., Jr. : Fas (CD95) expression is up-regulated on papillary thyroid carcinoma. J. Clin. Endocr. Metab. 84: 4246-4252, 1999. 3. Zhang, X.; Miao, X.; Sun, T.; Tan, W.; Qu, S.; Xiong, P.; Zhou, Y.; Lin, D. : Functional polymorphisms in cell death pathway genes FAS and FASL contribute to the risk of lung cancer. J. Med. Genet. 42: 479-484, 2005.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36835 MW

Observed Molecular Weight

12 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat , By Heat <br>Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat , -<br>Western blot, 0.1-0.5μg/ml, Mouse, Rat <br>