Anti-IFN gamma Antibody Picoband®, APC Conjugate

Boster Bio Anti-IFN gamma Antibody catalog # RP1001. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Synonyms

Interferon gamma;IFN-gamma;Ifng;

Gene Name

Interferon gamma

UniProt

P01581

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived rat IFN gamma recombinant protein (Position: 23-156).

Clonality

Polyclonal

Tissue Specificity

Released primarily from activated T lymphocytes.

Applications

ELISA,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Components

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

References & Citations

1. Awad, M.; Pravica, V.; Perrey, C.; El Gamel, A.; Yonan, N.; Sinnott, P. J.; Hutchinson, I. V. CA repeat allele polymorphism in the first intron of the human interferon-gamma gene is associated with lung allograft fibrosis. Hum. Immunol. 60: 343-346, 1999. 2. Nathan, C. F.; Murray, H. W.; Wiebe, M. E.; Rubin, B. Y. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J. Exp. Med. 158: 670-689, 1983. 3. Rossouw, M.; Nel, H. J.; Cooke, G. S.; van Helden, P. D.; Hoal, E. G. Association between tuberculosis and a polymorphic NF-kappa-B binding site in the interferon gamma gene. Lancet 361: 1871-1872, 2003. 4. Szabo, S. J.; Sullivan, B. M.; Stemmann, C.; Satoskar, A. R.; Sleckman, B. P.; Glimcher, L. H. Distinct effects of T-bet in T(H)1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells. Science 295: 338-342, 2002. 5. Zohlnhofer, D.; Richter, T.; Neumann, F.-J.; Nuhrenberg, T.; Wessely, R.; Brandl, R.; Murr, A.; Klein, C. A.; Baeuerle, P. A. Transcriptome analysis reveals a role of interferon-gamma in human neointima formation. Molec. Cell 7: 1059-1069, 2001.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.