Anti-RNF111 Antibody Picoband®, APC Conjugate

Boster Bio Anti-RNF111 Antibody Picoband® catalog # A05708-1. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

E3 ubiquitin-protein ligase Arkadia is an enzyme that in humans is encoded by the RNF111 gene. The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Gene Name

RNF111

Gene ID

54778

UniProt

Q6ZNA4

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

E.coli-derived human RNF111 recombinant protein (Position: F134-S994).

Clonality

Polyclonal

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Episkopou, V., Arkell, R., Timmons, P. M., Walsh, J. J., Andrew, R. L., Swan, D. Induction of the mammalian node requires Arkadia function in the extraembryonic lineages. Nature 410: 825-830, 2001. 2. Niederlander, C., Walsh, J. J., Episkopou, V., Jones, C. M. Arkadia enhances nodal-related signalling to induce mesendoderm. Nature 410: 830-834, 2001.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Applications Notes

Western blot, 0.25-0.5 μg/ml, Mouse Immunohistochemistry, 2-5 μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -